Published June 28, 1991
| public
Journal Article
Engineered metal-binding proteins: purification to protein folding
- Creators
-
Arnold, Frances H.
- Haymore, Barry L.
Abstract
Proteins can make use of metal ions to bind substrates, to maintain structure, to effect catalysis, and for allosteric control and regulation. In order to hold a particular metal ion with high affinity and specificity, proteins form multidentate binding pockets designed to fulfill both the chemical and geometric bonding requirements of that metal. Metal recognition can be engineered into proteins for applications such as protein purification.
Additional Information
© 1991 American Association for the Advancement of Science. We thank G. Wuenschell, R. Todd, S.-S. Suh, J. Kellis, R. Lcimgruber, G. Bild, and G. Krivi fur their contributions to this work. Support from Monsanto Company, the National Science Foundation, and the Office of Naval Research and a fellowship from the David and Lucile Packard Foundation are gratefully acknowledged (F.H.A.).Additional details
- Eprint ID
- 52811
- DOI
- 10.1126/science.1648261
- Resolver ID
- CaltechAUTHORS:20141215-105527216
- Monsanto Company
- NSF
- Office of Naval Research (ONR)
- David and Lucile Packard Foundation
- Created
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2014-12-15Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field