Lensfree optofluidic plasmonic sensor for real-time and label-free monitoring of molecular binding events over a wide field-of-view
Abstract
We demonstrate a high-throughput biosensing device that utilizes microfluidics based plasmonic microarrays incorporated with dual-color on-chip imaging toward real-time and label-free monitoring of biomolecular interactions over a wide field-of-view of >20 mm^2. Weighing 40 grams with 8.8 cm in height, this biosensor utilizes an opto-electronic imager chip to record the diffraction patterns of plasmonic nanoapertures embedded within microfluidic channels, enabling real-time analyte exchange. This plasmonic chip is simultaneously illuminated by two different light-emitting-diodes that are spectrally located at the right and left sides of the plasmonic resonance mode, yielding two different diffraction patterns for each nanoaperture array. Refractive index changes of the medium surrounding the near-field of the nanostructures, e.g., due to molecular binding events, induce a frequency shift in the plasmonic modes of the nanoaperture array, causing a signal enhancement in one of the diffraction patterns while suppressing the other. Based on ratiometric analysis of these diffraction images acquired at the detector-array, we demonstrate the proof-of-concept of this biosensor by monitoring in real-time biomolecular interactions of protein A/G with immunoglobulin G (IgG) antibody. For high-throughput on-chip fabrication of these biosensors, we also introduce a deep ultra-violet lithography technique to simultaneously pattern thousands of plasmonic arrays in a cost-effective manner.
Additional Information
© 2014 Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. Received 28 July 2014. Accepted 6 October 2014. Published 27 October 2014. Altug Research Group acknowledges National Science Foundation (NSF) CAREER Award, Presidential Early Career Award for Scientist and Engineers (PECASE) ECCS-0954790, Office of Naval Research Young Investigator Award 11PR00755-00-P00001, NSF Engineering Research Center on Smart Lighting EEC-0812056, Massachusetts Life Sciences Center Young Investigator award and Ecole Polytechnique Federale de Lausanne. Ozcan Research Group at UCLA gratefully acknowledges the support of the Presidential Early Career Award for Scientists and Engineers (PECASE), Army Research Office (ARO) Life Sciences Division (ARO; W911NF-13-1-0419 and W911NF-13-1-0197), ARO Young Investigator Award, National Science Foundation (NSF) CAREER Award, NSF CBET Division Biophotonics Program, NSF Emerging Frontiers in Research and Innovation (EFRI) Award, NSF EAGER Award, Office of Naval Research (ONR), the Howard Hughes Medical Institute (HHMI), and National Institutes of Health (NIH) Director's New Innovator Award DP2OD006427 from the Office of the Director, National Institutes of Health. This work is partially based upon research performed in a renovated laboratory by the National Science Foundation under Grant No. 0963183, which is an award funded under the American Recovery and Reinvestment Act of 2009 (ARRA). The authors also thank UCSB for their contribution in fabrication of the nanoapertures.Attached Files
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Additional details
- PMCID
- PMC4209447
- Eprint ID
- 52464
- Resolver ID
- CaltechAUTHORS:20141208-101947905
- ECCS-0954790
- NSF
- 11PR00755-00-P00001
- Office of Naval Research (ONR)
- EEC-0812056
- NSF
- Massachusetts Life Sciences Center
- Ecole Polytechnique Federale de Lausanne
- W911NF-13-1-0419
- Army Research Office (ARO)
- W911NF-13-1-0197
- Army Research Office (ARO)
- Howard Hughes Medical Institute (HHMI)
- DP2OD006427
- NIH
- 0963183
- American Recovery and Reinvestment Act of 2009 (ARRA)
- Created
-
2014-12-09Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field