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Published September 16, 1997 | Published
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Calx, a Na-Ca exchanger gene of Drosophila melanogaster

Schwarz, Erich M. ORCID icon
Benzer, Seymour
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Abstract

We have cloned Calx, a gene that encodes a Na-Ca exchanger of Drosophila melanogaster. Calx encodes two repeated motifs, Calx-α and Calx-β, that overlap domains required for exchanger activity and regulation. Calx has multiple transcripts in adults, including at least one expressed in the retina. The Calx genomic locus comprises ≥35 kb between the Atpα and rudimentary-like genes in chromosomal region 93B. In Xenopus oocytes, microinjected Calx cRNA induces calcium uptake like that of its homolog, the 3Na^+-1Ca^(2+) exchanger of mammalian heart. Implications of Calx-α motifs for the mechanism of Na-Ca exchange are discussed.

Additional Information

© 1997 by The National Academy of Sciences. Contributed by Seymour Benzer, July 9, 1997. We thank E. Eichenberger, L. Dowling, H. Davis, A. Gomez, and J. Gollub for technical assistance; the Indiana Drosophila Stock Center for Drosophila stocks; J. Tamkun, C. Labarca, and J. Rawls for genomic libraries and clones; N. Bonini for determining the chromosomal location of Calx; H. Lester and M. Quick for aiding our Xenopus expression work; D. Nicoll for NCX1 clones and reagents; members of the Benzer, Dreyer, and Lipshitz laboratories for cDNA libraries, protocols, and criticism; and K. Philipson for advice and encouragement. This work was supported by grants to S.B. from the National Science Foundation (MCB-9408718), the National Eye Institute (EY09278), the National Institute of Aging (AG12289), and the James G. Boswell Foundation, and by a grant to E.M.S. and S.B. from the Beckman Institute. Data deposition: The sequence reported in this paper has been deposited in the GenBank database (accession no. AF009897). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked ''advertisement'' in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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