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Published September 16, 1997 | Published
Journal Article Open

A possible role for kinase-associated protein phosphatase in the Arabidopsis CLAVATA1 signaling  pathway

Abstract

Continuous growth and development in plants are accomplished by meristems, groups of undifferentiated cells that persist as stem cells and initiate organs. While the structures of the apical and floral meristems in dicotyledonous plants have been well described, little is known about the underlying molecular mechanisms controlling cell proliferation and differentiation in these structures. We have shown previously that the CLAVATA1 (CLV1) gene in Arabidopsis encodes a receptor kinase-like protein that controls the size of the apical and floral meristems. Here, we show that KAPP, a gene encoding a kinase-associated protein phosphatase, is expressed in apical and young floral meristems, along with CLV1. Overexpression of KAPP mimics the clv1 mutant phenotype. Furthermore, CLV1 has kinase activity: it phosphorylates both itself and KAPP. Finally, KAPP binds and dephosphorylates CLV1. We present a model where KAPP functions as a negative regulator of the CLAVATA1 signal transduction pathway.

Additional Information

© 1997 The National Academy of Sciences. Contributed by Elliot M. Meyerowitz, July 23, 1997. We thank Hajime Sakai for assistance with the in situ hybridizations, Doris Wagner for advice on protein purification, and Akiko Kumagai for assistance with the phosphoamino acid analysis. We acknowledge Ray Deshaies and Renny Feldman for discussions, comments, and reagents. We also thank Xuemei Chen, Bill Dunphy, Jennifer Fletcher, Jian Hua, Toshiro Ito, Steve Jacobsen, Carolyn Ohno, Jose-Luis Reichmann, Mark Running, Robert Sablowski, Hajime Sakai, Tom Tubman, and Eva Ziegelhoffer for careful review of this manuscript. This work was supported by National Science Foundation Grant MCB-9204839 and a Strategic Research Fund Grant from Zeneca Seeds (to E.M.M). R.W.W. was supported by National Institutes of Health Predoctoral Training Grant GM07616 and the Howard Hughes Medical Institute. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked ''advertisement'' in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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