Published July 1, 2005
| Supplemental Material
Journal Article
Open
Long-Term Monitoring of Bacteria Undergoing Programmed Population Control in a Microchemostat
Chicago
An error occurred while generating the citation.
Abstract
Using an active approach to preventing biofilm formation, we implemented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small populations of bacteria with single-cell resolution. We used this device to observe the dynamics of Escherichia coli carrying a synthetic "population control" circuit that regulates cell density through a feedback mechanism based on quorum sensing. The microfluidic bioreactor enabled long-term monitoring of unnatural behavior programmed by the synthetic circuit, which included sustained oscillations in cell density and associated morphological changes, over hundreds of hours.
Additional Information
© 2005 American Association for the Advancement of Science. Received 27 December 2004; accepted 10 May 2005. We thank M. Elowitz for MC4100Z1 cells, U. Alon for MG1655 cells, T. Ozdere for generating data for figs. S4 and S5, C. Collins for plasmid pLuxR, M. Barnett for technical assistance, and J. Leadbetter, C. Ward, T. Squires, J. Huang, and E. Kartalov for helpful discussions. Supported in part by the NSF and the Defense Advanced Research Projects Agency (contract no. N66001-02-1-8929).Attached Files
Supplemental Material - balagadde-SOM.pdf
Files
balagadde-SOM.pdf
Files
(7.3 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:e1b3aa65fbbc508398511687ae98d6b7
|
7.3 MB | Preview Download |
Additional details
- Eprint ID
- 52041
- Resolver ID
- CaltechAUTHORS:20141121-103618066
- NSF
- Defense Advanced Research Projects Agency (DARPA)
- N66001-02-1-8929
- Created
-
2014-11-21Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field