A Synthetic Maternal-Effect Selfish Genetic Element Drives Population Replacement in Drosophila

Creators: Chen, Chun-Hong; Huang, Haixia; Ward, Catherine M.; Su, Jessica T.; Schaeffer, Lorian V.; Guo, Ming; Hay, Bruce A.

Style

An error occurred while generating the citation.

Abstract

One proposed strategy for controlling the transmission of insect-borne pathogens uses a drive mechanism to ensure the rapid spread of transgenes conferring disease refractoriness throughout wild populations. Here, we report the creation of maternal-effect selfish genetic elements in Drosophila that drive population replacement and are resistant to recombination-mediated dissociation of drive and disease refractoriness functions. These selfish elements use microRNA-mediated silencing of a maternally expressed gene essential for embryogenesis, which is coupled with early zygotic expression of a rescuing transgene.

Additional Information

© 2007 American Association for the Advancement of Science. 8 December 2006; Accepted 20 March 2007; Published online 29 March 2007. This work did not receive specific funding. It was supported by NIH grants GM057422 and GM70956 to B.A.H. and NS042580 and NS048396 to M.G. C.-H.C. was supported by the Moore Foundation Center for Biological Circuit Design. We thank two reviewers for useful comments and improvements on the manuscript. GenBank accession numbers for Medea^(myd88) and Medea^(myd88-int) are EF447106 and EF447105, respectively.

Attached Files

Supplemental Material - Chen.SOM.pdf

Files

Chen.SOM.pdf

Files (337.1 kB)

Name	Size	Download all
Chen.SOM.pdf md5:b651c3573fd1ca0535e12545b8de34bc	337.1 kB	Preview Download

Additional details

	All versions	This version
Views	0	0
Downloads	0	0
Data volume	0 Bytes	0 Bytes