Kinetics of Morphogen Gradient Formation
Abstract
In the developing fly wing, secreted morphogens such as Decapentaplegic (Dpp) and Wingless (Wg) form gradients of concentration providing positional information. Dpp forms a longer-range gradient than Wg. To understand how the range is controlled, we measured the four key kinetic parameters governing morphogen spreading: the production rate, the effective diffusion coefficient, the degradation rate, and the immobile fraction. The four parameters had different values for Dpp versus Wg. In addition, Dynamin-dependent endocytosis was required for spreading of Dpp, but not Wg. Thus, the cellular mechanisms of Dpp and Wingless spreading are different: Dpp spreading requires endocytic, intracellular trafficking.
Additional Information
© 2007 American Association for the Advancement of Science. 2 October 2006; Accepted 6 December 2006. We thank J.-P. Vincent and A. Charpilienne for reagents, C. P. Heisenberg and E. Entchev for critical reading of the manuscript, as well as all members of the M.G.-G. and F.J. groups. This work was supported by the Max Planck Society, Deutsche Forchungsgemeinschaft, Volkswagen Stiftung, the European Union, and the Human Frontier Science Program.Attached Files
Supplemental Material - 1125774S1.mov
Supplemental Material - 1125774S2.mov
Supplemental Material - 1125774S3.mov
Supplemental Material - Kicheva-SOM-5811.pdf
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Additional details
- Eprint ID
- 51966
- Resolver ID
- CaltechAUTHORS:20141119-121008388
- Max Planck Society
- Deutsche Forschungsgemeinschaft (DFG)
- Volkswagen Stiftung
- European Union
- Human Frontier Science Program
- Created
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2014-11-19Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field