Cell Dynamics During Somite Boundary Formation Revealed by Time-Lapse Analysis
- Creators
- Kulesa, Paul M.
-
Fraser, Scott E.
Abstract
We follow somite segmentation in living chick embryos and find that the shaping process is not a simple periodic slicing of tissue blocks but a much more carefully choreographed separation in which the somite pulls apart from the segmental plate. Cells move across the presumptive somite boundary and violate gene expression boundaries thought to correlate with the site of the somite boundary. Similarly, cells do not appear to be preassigned to a given somite as they leave the node. The results offer a detailed picture of somite shaping and provide a spatiotemporal framework for linking gene expression with cell movements.
Additional Information
© 2002 American Association for the Advancement of Science. 27 June 2002; accepted28 August 2002. We thank M. Dickinson andJ. Kastner for comments on the manuscript andhelp with in situ hybridizations, and A. Ewald, H. McBride, M. Reddington, and R. Kerschman for their help with the serial sectioning by surface imaging microscopy (30). P.M.K. is a participant in the California Institute of Technology Initiative in Computational Molecular Biology, which is funded by a Burroughs Wellcome Fund Interfaces award.Attached Files
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Supplemental Material - 1075544s2.mov
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Supplemental Material - suppmat.kules.pdf
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Additional details
- Eprint ID
- 51948
- DOI
- 10.1126/science.1075544
- Resolver ID
- CaltechAUTHORS:20141119-094303997
- Burroughs Wellcome Fund Interfaces Award
- Created
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2014-11-19Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field