The Opisthorchis viverrini genome provides insights into life in the bile duct
- Creators
- Young, Neil D.
- Nagarajan, Niranjan
- Lin, Suling Joyce
- Korhonen, Pasi K.
- Jex, Aaron R.
- Hall, Ross S.
- Safavi-Hemami, Helena
- Kaewkong, Worasak
- Bertrand, Denis
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Gao, Song
- Seet, Qihui
- Wongkham, Sopit
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Teh, Bin Tean
- Wongkham, Chaisiri
- Intapan, Pewpan Maleewong
- Maleewong, Wanchai
- Yang, Xinhua
- Hu, Min
- Wang, Zuo
- Hofmann, Andreas
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Sternberg, Paul W.
- Tan, Patrick
- Wang, Jun
- Gasser, Robin B.
Abstract
Opisthorchiasis is a neglected, tropical disease caused by the carcinogenic Asian liver fluke, Opisthorchis viverrini. This hepatobiliary disease is linked to malignant cancer (cholangiocarcinoma, CCA) and affects millions of people in Asia. No vaccine is available, and only one drug (praziquantel) is used against the parasite. Little is known about O. viverrini biology and the diseases that it causes. Here we characterize the draft genome (634.5 Mb) and transcriptomes of O. viverrini, elucidate how this fluke survives in the hostile environment within the bile duct and show that metabolic pathways in the parasite are highly adapted to a lipid-rich diet from bile and/or cholangiocytes. We also provide additional evidence that O. viverrini and other flukes secrete proteins that directly modulate host cell proliferation. Our molecular resources now underpin profound explorations of opisthorchiasis/CCA and the design of new interventions.
Additional Information
© 2014 The Authors. This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. Received 3 March 2014; Accepted 11 June 2014; Published 9 July 2014. We thank the staff of BGI-Shenzhen for their contributions. This project was funded by the Australian Research Council, the National Health and Medical Research Council (NHMRC) of Australia and BGI-Shenzhen (R.B.G.). Other support from the Alexander von Humboldt Foundation, Melbourne Water Corporation (R.B.G.) and Genome Institute of Singapore (P.T.) is gratefully acknowledged. This project was also supported by a Victorian Life Sciences Computation Initiative (grant number VR0007) on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government. N.D.Y. holds an NHMRC Early Career Research Fellowship. P.W.S. thanks the Howard Hughes Medical Institute (HHMI) and the National Institutes of Health (NIH). We would specifically like to acknowledge the research scientists that developed the programs used in this study. Given restrictions on the number of publications that could be cited, we were unable to include all original articles in the methods section. Instead, we have included links to their respective websites. This paper is dedicated to the memory of Eduard Gasser.Attached Files
Published - ncomms5378.pdf
Supplemental Material - ncomms5378-s1.pdf
Supplemental Material - ncomms5378-s2.xlsx
Supplemental Material - ncomms5378-s3.xlsx
Supplemental Material - ncomms5378-s4.xlsx
Supplemental Material - ncomms5378-s5.xlsx
Supplemental Material - ncomms5378-s6.xlsx
Supplemental Material - ncomms5378-s7.xlsx
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Additional details
- PMCID
- PMC4104445
- Eprint ID
- 49646
- Resolver ID
- CaltechAUTHORS:20140912-092833842
- Australian Research Council
- National Health and Medical Research Council (NHMRC)
- BGI-Shenzhen
- Alexander von Humboldt Foundation
- Melbourne Water Corporation
- Genome Institute of Singapore
- Victorian Life Sciences Computation Initiative
- VR0007
- Howard Hughes Medical Institute (HHMI)
- NIH
- Created
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2014-09-12Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field