A novel HoxB cluster protein expressed in the hindbrain and pharyngeal arches
- Creators
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Saxena, Ankur
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Bronner, Marianne E.
Abstract
We describe a novel zebrafish line that fluorescently tags a previously unknown protein, CT74a, allowing us to follow its endogenous expression in real time and at subcellular resolution in live embryos. Our results showed that CT74a-Citrine fusion protein is expressed in the developing pharyngeal arches, hindbrain, and fin buds in a pattern highly reminiscent of transcription factors belonging to anterior Hox gene families, including expression in a subset of neuronal nuclei. Consistent with this, splinkerette-PCR revealed that CT74a-Citrine's genomic integration is within the HoxB region, and 3′ RACE demonstrated that its downstream coding sequence has no recognizable homology. Thus, CT74a is a previously unknown protein located within the HoxB cluster adjacent to Hoxb4a and is expressed in a Hoxb4a-like pattern.
Additional Information
© 2014 Wiley Periodicals, Inc. Received 5 July 2014; Revised 11 August 2014; Accepted 13 August 2014. Article first published online: 23 AUG 2014. We thank David Mayorga for zebrafish husbandry assistance; Ilana Solomon for technical support; Dr. Hugo Parker for scientific feedback; Dr. Bruce Appel for the Sox10:mRFP line; Dr. Uwe Strahle for the Ngn1:nRFP line.Attached Files
Accepted Version - nihms-623261.pdf
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Additional details
- PMCID
- PMC4211940
- Eprint ID
- 48835
- DOI
- 10.1002/dvg.22806
- Resolver ID
- CaltechAUTHORS:20140825-090002879
- NIH
- P50HG004071
- Gordon Ross Postdoctoral Fellowship
- NIH
- 5T32NS007251
- Created
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2014-08-25Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field