Spatial Expression of the Alternatively Spliced EIIIB and EIIIA Segments of Fibronectin in the Early Chicken Embryo
Abstract
Using domain-specific antibodies, we have analyzed the tissue distribution of fibronectins (FNs) containing the alternatively spliced EIIIB and EIIIA segments relative to total FN in early chicken embryos. The results show a selective loss of EIIIA+ FN staining in the notochordal sheath and in cartilaginous structures between 4.5 and 7.0 days of development. In other regions, EIIIB+ and EIIIA+ FNs are extensively codistributed in and around mesoderm-derived structures (somites, notochord, heart, and blood vessels), in basal laminae of endoderm and ectoderm-derived structures, as well as within the vicinity of neural crest formation and migration. We also noted that EIIIA staining overlaps with spatial patterns of distribution that have previously been described for the α4 integrin subunit, a component of the EIIIA receptor α4β1.
Additional Information
© 2002 Taylor and Francis. Received 29 July 2002; accepted 26 November 2002. JHP was supported by a UCLA Claude Pepper Older Americans Independence Center, NIA #P60 AG10415, a gift from the Charles B. See Foundation, as well as Career Development Award and Merit Review Grants, both from the Department of Veterans Affairs. MBF was supported by USPHS HD15527. We wish to thank Richard Hynes for generous gifts of antibodies.Additional details
- Eprint ID
- 47414
- DOI
- 10.1080/15419060216015
- Resolver ID
- CaltechAUTHORS:20140723-083018918
- UCLA Claude Pepper Older Americans Independence Center
- P60 AG10415
- NIH
- Charles B. See Foundation
- Department of Veterans Affairs
- HD15527
- U.S. Public Health Service (USPHS)
- Created
-
2014-07-23Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field