Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published November 1, 2013 | Supplemental Material
Journal Article Open

Molecular Cloning, Expression, and In Silico Structural Analysis of Guinea Pig IL-17

Abstract

Interleukin-17A (IL-17A) is a potent proinflammatory cytokine and the signature cytokine of Th17 cells, a subset which is involved in cytokine and chemokine production, neutrophil recruitment, promotion of T cell priming, and antibody production. IL-17 may play an important role in tuberculosis and other infectious diseases. In preparation for investigating its role in the highly relevant guinea pig model of pulmonary tuberculosis, we cloned guinea pig IL-17A for the first time. The complete coding sequence of the guinea pig IL-17A gene (477 nucleotides; 159 amino acids) was subcloned into a prokaryotic expression vector (pET-30a) resulting in the expression of a 17 kDa recombinant guinea pig IL-17A protein which was confirmed by mass spectrometry analysis. Homology modeling of guinea pig IL-17A revealed that the three-dimensional structure resembles that of human IL-17A. The secondary structure predicted for this protein showed the presence of one extra helix in the N-terminal region. The expression profile of IL-17A was analyzed quantitatively in spleen, lymph node, and lung cells from BCG-vaccinated guinea pigs by real-time PCR. The guinea pig IL-17A cDNA and its recombinant protein will serve as valuable tools for molecular and immunological studies in the guinea pig model of pulmonary TB and other human diseases.

Additional Information

© 2013 Springer Science+Business Media New York. Published online: 29 June 2013. This work was supported by a subcontract from Colorado State University under NIH contract HHSN 266200400091c. The authors thank Dr. Larry Dangott of the Protein Chemistry Lab at Texas A&M University for mass spectrometry analysis.

Attached Files

Supplemental Material - Fig1.pptx

Supplemental Material - Fig2.pptx

Supplemental Material - Fig3.pptx

Supplemental Material - Fig4.pptx

Supplemental Material - Fig5.pptx

Files

Files (1.9 MB)
Name Size Download all
md5:0a44879b4d1b3a6b62afbe11c78e79f3
324.5 kB Download
md5:cbf17df0084da187bc1ee2d9beacfe5e
156.7 kB Download
md5:460a8726ab346b790e9d065d0924a997
193.8 kB Download
md5:fb17556dd5fe1249c0b3a08680c5c1e7
298.3 kB Download
md5:221819a48dc0858f05065094b2e32c7d
928.6 kB Download

Additional details

Created:
August 22, 2023
Modified:
October 25, 2023