Electrochemical Patterning and Detection of DNA Arrays on a Two-Electrode Platform
Abstract
We report a novel method of DNA array formation that is electrochemically formed and addressed with a two-electrode platform. Electrochemical activation of a copper catalyst, patterned with one electrode, enables precise placement of multiple sequences of DNA onto a second electrode surface. The two-electrode patterning and detection platform allows for both spatial resolution of the patterned DNA array and optimization of detection through DNA-mediated charge transport with electrocatalysis. This two-electrode platform has been used to form arrays that enable differentiation between well-matched and mismatched sequences, the detection of TATA-binding protein, and sequence-selective DNA hybridization.
Additional Information
© 2013 American Chemical Society. Received: October 24, 2013; Publication Date (Web): December 12, 2013. We are grateful to the NIH (GM61077) and the Arnold and Mabel Beckman Foundation for support of this work. The authors declare no competing financial interest.Attached Files
Accepted Version - nihms553677.pdf
Supplemental Material - ja410902j_si_001.pdf
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Additional details
- PMCID
- PMC4026266
- Eprint ID
- 43139
- Resolver ID
- CaltechAUTHORS:20131223-100408108
- NIH
- GM61077
- Arnold and Mabel Beckman Foundation
- Created
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2013-12-23Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field