Hemoglobin Synthesis in Rabbit Reticulocytes In vitro
- Creators
- Kruh, Jacques
- Borsook, Henry
Abstract
We have reported the rapid incorporation of labeled amino acids into the proteins of rabbit reticulocytes in vitro and the stimulation of this process by certain amino acids, iron, fructose-amino acids, glucose, and some, as yet, unidentified material in the filtrate of boiled plasma. The incorporation was measured in the mixture of the total proteins of the reticulocytes (1, 2). In the study reported here two protein fractions were isolated, the hemoglobin, which comprises more than 75 per cent of the total, and the water-insoluble proteins. The hemoglobin was fractionated into heme and globin. Experiments were carried out with four C^(14)-labeled amino acids and with different stimulators to ascertain whether heme synthesis and amino acid incorporation went parallel or not.
Additional Information
© 1955 American Society for Biochemistry and Molecular Biology. Received for publication, September 19, 1955. This study was aided by a contract between the Atomic Energy Commission and the Division of Biology, California Institute of Technology. It was also supported by a research grant from the National Institutes of Health, United States Public Health Service, and by a grant-in-aid from the American Cancer Society upon recommendation of the Committee on Growth of the National Research Council.Attached Files
Published - KRUjbc56.pdf
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Additional details
- Eprint ID
- 42956
- Resolver ID
- CaltechAUTHORS:20131211-111009191
- Atomic Energy Commission
- NIH
- United States Public Health Service (USPHS)
- American Cancer Society
- Created
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2013-12-11Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field