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Published September 1, 2013 | Supplemental Material + Published
Journal Article Open

Transcriptome Profiling of Nasonia vitripennis Testis Reveals Novel Transcripts Expressed from the Selfish B Chromosome, Paternal Sex Ratio

Abstract

A widespread phenomenon in nature is sex ratio distortion of arthropod populations caused by microbial and genetic parasites. Currently little is known about how these agents alter host developmental processes to favor one sex or the other. The paternal sex ratio (PSR) chromosome is a nonessential, paternally transmitted centric fragment that segregates in natural populations of the jewel wasp, Nasonia vitripennis. To persist, PSR is thought to modify the hereditary material of the developing sperm, with the result that all nuclear DNA other than the PSR chromosome is destroyed shortly after fertilization. This results in the conversion of a fertilized embryo—normally a female—into a male, thereby insuring transmission of the "selfish" PSR chromosome, and simultaneously leading to wasp populations that are male-biased. To begin to understand this system at the mechanistic level, we carried out transcriptional profiling of testis from WT and PSR-carrying males. We identified a number of transcripts that are differentially expressed between these conditions. We also discovered nine transcripts that are uniquely expressed from the PSR chromosome. Four of these PSR-specific transcripts encode putative proteins, whereas the others have very short open reading frames and no homology to known proteins, suggesting that they are long noncoding RNAs. We propose several different models for how these transcripts could facilitate PSR-dependent effects. Our analyses also revealed 15.71 MB of novel transcribed regions in the N. vitripennis genome, thus increasing the current annotation of total transcribed regions by 53.4%. Finally, we detected expression of multiple meiosis-related genes in the wasp testis, despite the lack of conventional meiosis in the male sex.

Additional Information

© 2013 Akbari et al. Received May 24, 2013. Accepted July 16, 2013. Communicating editor: S. R. Hawley. Data from this article have been deposited with the EMBL/GenBank Data Libraries under SRA accession nos. WT: SRX325702 (WT) and SRX325704 (PSR); corresponding BioSamples: SRS462062 (WT); SRS462063 (PSR); BioProject: SRP027543.

Attached Files

Published - 1597.full.pdf

Supplemental Material - 007583SI.pdf

Supplemental Material - FileS1.zip

Supplemental Material - FileS2.zip

Supplemental Material - FileS3.zip

Supplemental Material - FileS4.zip

Supplemental Material - FileS5.pdf

Supplemental Material - TableS1.xlsx

Supplemental Material - TableS10.xlsx

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Supplemental Material - TableS5.xlsx

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Supplemental Material - TableS8.xlsx

Supplemental Material - TableS9.xlsx

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Additional details

Created:
August 19, 2023
Modified:
October 24, 2023