Published October 11, 2013
| public
Journal Article
Selective Functionalization of the Protein N Terminus with N-Myristoyl Transferase for Bioconjugation in Cell Lysate
Abstract
A site to behold: Robust site-specific functionalization of engineered proteins is achieved with N-myristoyl transferase (NMT) in bacterial cells. NMT tolerates non-natural substrate proteins as well as reactive fatty acid tags, rendering it a powerful tool for protein conjugation applications, including the construction of protein microarrays from lysate.
Additional Information
© 2013 Wiley-VCH Verlag GmbH& Co. KGaA, Weinheim. Received: July 11, 2013. Article first published online: 12 Sep. 2013. We thank John Ngo, Rebecca Connor, David Ralin, Sandy Tungteakkhun, and Claudia Lee for helpful discussions, and Mona Shahgoli and Robert Graham for assistance with mass spectrometry studies. Support for this work was provided by the Jacobs Institute for Molecular Engineering for Medicine and the Caltech Innovation Initiative.Additional details
- Eprint ID
- 41424
- DOI
- 10.1002/cbic.201300453
- Resolver ID
- CaltechAUTHORS:20130919-145751587
- Jacobs Institute for Molecular Engineering for Medicine
- Caltech Innovation Initiative
- Created
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2013-09-20Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field