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Published December 11, 2006 | Accepted Version
Journal Article Open

Time-Controlled Microfluidic Seeding in nL-Volume Droplets To Separate Nucleation and Growth Stages of Protein Crystallization

Abstract

This paper describes a method of time-controlled seeding to separate the stages of nucleation and growth in protein crystallization using a microfluidic device.

Additional Information

Copyright © 2006 WILEY-VCH. Issue published online: 4 DEC 2006. Article first published online: 13 NOV 2006. Manuscript Revised: 12 SEP 2006. Manuscript Received: 22 JUL 2006. This work was supported by NIH Protein Structure Initiative Specialized Centers Grant GM074961 (ATCG3D) and the NIH (R01 EB001903). Use of the Advanced Photon Source was supported by the US Department of Energy (contract no. W-31–109-Eng-38). Use of the BioCARS Sector 14 was supported by the NIH National Center for Research Resources (grant number RR07707). GM/CA-CAT has been funded in whole or in part by the National Cancer Institute (Y1-CO-1020) and the National Institute of General Medical Sciences (Y1-GM-1104). Funding for functional and structural proteomics of SARS CoV-related proteins is provided through NIH-NIAID contract HHSN266200400058C. We thank Ruslan Sanishvili (GM/CA Cat station 23ID-D staff support) for technical assistance; Scott Lovell and Lance Stewart (deCODE Biostructures) for helpful assistance and discussions; Shu Moy (Midwest Center for Structural Genomics) for cloning work on Oligoendopeptidase F; Vanitha Subramanian (The Scripps Research Institute) for cloning, expression, and purification of SARS nucleocapsid N-terminal domain; and L. Spencer Roach (University of Chicago) for help with thaumatin X-ray diffraction comparisons.

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Additional details

Created:
August 19, 2023
Modified:
October 24, 2023