Published July 13, 2013
| Accepted Version
Journal Article
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Kinetics of CO recombination to the heme in Geobacillus stearothermophilus nitric oxide synthase
Chicago
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Abstract
We report the kinetics of CO rebinding to the heme in His134Ser, Ile223Val and His134Ser/Ile223Ser mutants of Geobacillus stearothermophilus nitric oxide synthase (gsNOS). The amplitudes of the two observed kinetics phases, which are insensitive to CO concentration, depend on enzyme concentration. We suggest that two forms of gsNOS are in equilibrium under the conditions employed (6.1–27 μM gsNOS with 20 or 100% CO atmosphere). The kinetics of CO rebinding to the heme do not depend on the identity of the NO-gate residues at positions 134 and 223.
Additional Information
© 2012 Elsevier Ltd. Available online 21 September 2012. Our work was supported by NIH (DK019038 to H.B.G. and J.R.W.) and (GM095037 to J.J.W.).Attached Files
Accepted Version - nihms409341.pdf
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Additional details
- PMCID
- PMC3747573
- Eprint ID
- 39936
- DOI
- 10.1016/j.poly.2012.08.079
- Resolver ID
- CaltechAUTHORS:20130815-084001492
- NIH
- DK019038
- NIH
- GM095037
- Created
-
2013-08-16Created from EPrint's datestamp field
- Updated
-
2021-11-09Created from EPrint's last_modified field