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Published 2013 | public
Book Section - Chapter

Efficient Sampling of SCHEMA Chimera Families to Identify Useful Sequence Elements

Abstract

SCHEMA structure-guided recombination is an effective method for producing families of protein chimeras having high sequence diversity, functional diversity, and thermostabilities greater than any of the parent proteins from which the chimeras are made. A key feature of SCHEMA chimera families is their amenability to a "sample, model, and predict" operation that allows one to characterize members of a small chimera sample set and use those data to construct models that accurately predict the properties of every member of the family. In this chapter, we describe applications of this "sample, model, and predict" approach and outline methods for designing chimera sample sets that enable efficient construction of models to identify useful sequence elements. With these models we can also predict the sequences and properties of the most desirable chimeras.

Additional Information

© 2013 Elsevier Inc. The authors acknowledge funding from the Institute of General Medical Sciences of the National Institutes of Health (ARRA grant 2R01-GM068664-05A1) for work on cytochrome P450s and the U.S. Army Research Office Institute for Collaborative Biotechnologies (grant W911NF-09-D-0001) for technology development and cellulase engineering. The contents of this chapter are solely the responsibility of the authors and do not necessarily represent the official views of the sponsors.

Additional details

Created:
August 22, 2023
Modified:
January 13, 2024