Theoretical and Experimental Dissection of DNA Loop-Mediated Repression
Abstract
Transcriptional networks across all domains of life feature a wide range of regulatory architectures. Theoretical models now make clear predictions about how key parameters describing those architectures modulate gene expression, and the ability to construct genetic circuits with tunable parameters enables precise tests of such models. We dissect gene regulation through DNA looping by tuning network parameters such as repressor copy number, DNA binding strengths, and loop length in both thermodynamic models and experiments. Our results help clarify the short-length mechanical properties of DNA.
Additional Information
© 2013 American Physical Society. Received 30 August 2012; published 2 January 2013. This work was supported by the National Institutes of Health Pioneer Award No. DP1 OD000217 (H. G. G., R. P.) and Grants No. R01 GM085286 and No. R01 GM085286- 01S (J. Q. B., H. G. G., R. P.). We would like to acknowledge Jane Kondev, Jeff Gelles, Jim Maher, Jason Kahn, John Marko, Sunney Xie, Eran Segal, Sankar Adhya, Paul Wiggins, Thomas Kuhlman, David Bensimon, JonWidom, and Stephanie Johnson for many thoughtful discussions. J. Boedicker and H. Garcia contributed equally to this work.Attached Files
Published - PhysRevLett.110.018101.pdf
Accepted Version - nihms-492741.pdf
Supplemental Material - LV13637_Boedicker_PRL_looping_Suppl_Mat_revised_2.pdf
Supplemental Material - README.TXT
Files
Additional details
- PMCID
- PMC3716456
- Eprint ID
- 36775
- Resolver ID
- CaltechAUTHORS:20130205-103824324
- DP1 OD000217
- NIH
- R01 GM085286
- NIH
- 01 GM085286- 01S
- NIH
- Created
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2013-02-05Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field