A novel FoxD3 gene trap line reveals neural crest precursor movement and a role for FoxD3 in their specification
Abstract
Neural crest cells migrate extensively and contribute to diverse derivatives, including the craniofacial skeleton, peripheral neurons and glia, and pigment cells. Although several transgenic lines label neural crest subpopulations, few are suited for studying early events in neural crest development. Here, we present a zebrafish gene/protein trap line gt(foxd3-citrine)^(ct110a) that expresses a Citrine fusion protein with FoxD3, a transcription factor expressed in premigratory and migrating neural crest cells. In this novel line, citrine expression exactly parallels endogenous foxd3 expression. High-resolution time-lapse imaging reveals the dynamic phases of precursor and migratory neural crest cell movements from the neural keel stage to times of active cell migration. In addition, Cre-recombination produces a variant line FoxD3-mCherry-pA whose homozygosis generates a FoxD3 mutant. Taking advantage of the endogenously regulated expression of FoxD3-mCherry fusion protein, we directly assess early effects of FoxD3 loss-of-function on specification and morphogenesis of dorsal root ganglia, craniofacial skeleton and melanophores. These novel lines provide new insights and useful new tools for studying specification, migration and differentiation of neural crest cells.
Additional Information
© 2012 Elsevier Inc. Available online 8 December 2012. We thank L. Trinh, T. Sauka-Spengler and M. Simões-Costa for comments on the manuscript and helpful discussions, Tom Schilling for sharing foxd3 probe and Tg(FoxD3:EGFP) line, Leigh Ann Fletcher for fish care, Ilana Solomon, Neha Das and Joanne Tan for technical support and Daniel Koo for help with vector analysis. T.H.-H. was supported by a Pew Latin American Fellowship in Biomedical Sciences and by California Institute for Regenerative Medicine Training Grant (T2-00006). This work was supported by NIH grants HG004071 and HD037105 (M.E.B).Attached Files
Accepted Version - nihms585091.pdf
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Additional details
- PMCID
- PMC3553214
- Eprint ID
- 36296
- DOI
- 10.1016/j.ydbio.2012.11.035
- Resolver ID
- CaltechAUTHORS:20130110-101011580
- Pew Latin American Fellowship in Biomedical Sciences
- T2-00006
- California Institute for Regenerative Medicine (CIRM)
- HG004071
- NIH
- HD037105
- NIH
- Created
-
2013-01-10Created from EPrint's datestamp field
- Updated
-
2021-11-09Created from EPrint's last_modified field