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Published December 1, 2006 | Supplemental Material
Journal Article Open

Gene families encoding transcription factors expressed in early development of Strongylocentrotus purpuratus

Abstract

All genes encoding transcription factors of the bHLH, Nuclear Receptor, Basic Leucine Zipper, T-box, Smad, Sox, and other smaller families were identified in the Strongylocentrotus purpuratus genome by means of a permissive blast search of the genome using a database of known transcription factors. Phylogenetic trees were constructed for the major families, permitting a comparison of the regulatory protein repertoire of the sea urchin and other species. QPCR and whole mount in situ hybridization experiments revealed the temporal and spatial expression patterns of these genes during early development. These regulatory genes are initially expressed at a broad range of time points, and the large majority of genes of all families are expressed within the first 48 h of development. The observations suggest assignment of many regulatory genes to specific developmental sub-networks, including endomesodermal, oral, aboral, and apical.

Additional Information

© 2006 Elsevier Inc. Received for publication 1 June 2006; revised 5 August 2006; accepted 16 August 2006. Available online 22 August 2006. The authors would like to acknowledge Rachel Gray for her invaluable assistance in performing in situs; Jongmin Nam for advice and assistance in generating phylogenetic trees; Jed Goldstone and Mark Hahn for their advice on nuclear receptor phylogeny; and Deanna Thomas for her assistance with figures.

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