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Published November 1962 | Published
Journal Article Open

Staphylococcal Transducing Particle

Abstract

When novobiocin-resistant transductants were isolated under conditions that permitted superinfection, almost all the clones were lysogenic for the transducing phage. If superinfection was prevented, then the transductants isolated were nonlysogenic, suggesting the defective nature of the transducing particle. It was noted that the transducing and plaque-forming particles showed no appreciable difference in buoyant density. No difference was found in transduction rates when either sensitive or lysogenic cells were used as recipients. Transduction rates as high as one transductant per 7 × 10^4 phage particles were obtained for novobiocin resistance.

Additional Information

© 1962 The Williams & Wilkins Company. Received for publication June 25, 1962. This investigation was supported by a grant (E-3093) from the National Institutes of Health, U.S. Public Health Service; the senior author was a predoctoral fellow of the National Institutes of Health.

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Created:
August 19, 2023
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