Published February 1969
| Published
Journal Article
Open
Neurospora Mutant Exhibiting Hyperproduction of Amylase and Invertase
- Creators
- Gratzner, Howard
- Sheehan, D. N.
Abstract
A mutant strain of Neurospora crassa has been isolated which is derepressed for amylase and β-fructofuranosidase (invertase). Large amounts of the two enzymes were secreted into the culture medium upon depletion of exogenous carbon source. The resulting increases of the two extracellular enzymes were prevented by actinomycin D, cycloheximide, and glycerol. The starving cells of the mutant strain produced amylase and invertase de novo, as evidenced by incorporation of radioactive amino acids into the enzymes. Preliminary genetic studies indicate that these elevated enzyme levels described are due to a single gene mutation.
Additional Information
© 1969 American Society for Microbiology. Received for publication 1 November 1968. This investigation was supported by National Science Foundation grant GB-4537 and by a special Public Health Service fellowship, IF3-CA-25,17301. We are grateful for the very able assistance of Stephen Bloom.Attached Files
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Additional details
- PMCID
- PMC249725
- Eprint ID
- 33256
- Resolver ID
- CaltechAUTHORS:20120816-093814786
- GB-4537
- NSF
- IF3-CA-25,17301
- NIH
- Created
-
2012-08-16Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field