Positioning Flagellar Genes in Escherichia coli by Deletion Analysis
- Creators
- Silverman, Michael
- Simon, Melvin
Abstract
Two methods were devised to select a series of overlapping deletion mutations carried on episomal elements in Escherichia coli. The deletions were then used in an analysis of (i) the relative position on the genome of previously described mutant loci in the flagellar genes, (ii) the relative position of a newly defined cistron, flaN, and (iii) the orientation and direction of transcription of genes previously assigned to multicistronic transcriptional units. As a result of this analysis and previous work, we report the following arrangement of the flagellar genes in the his-aroD region of the E. coli genetic map: his-flaR-flaQ-flaP-flaA-flaE- flaO-flaC-flaB-flaN-hag-flaD-uvrC-flaI-mot-flaG-flaH. The genes flaA, P, Q, and R, flaB, C, 0 and E, and flaG and H are co-transcribed in that order.
Additional Information
© 1974 American Society for Microbiology. Received for publication 27 September 1973. The cooperation of Julius Adler in providing the nonchemotactic strain is appreciated. M. Silverman was supported by funds from the Cancer Coordinating Committee of the University of California, San Diego. This investigation was supported by a research grant, GB-15655, from the National Science Foundation.Attached Files
Published - SILjbact74c.pdf
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Additional details
- PMCID
- PMC246526
- Eprint ID
- 32909
- Resolver ID
- CaltechAUTHORS:20120803-095603378
- GB-15655
- NSF
- University of California, San Diego
- Created
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2012-08-03Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field