Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published February 1980 | Published
Journal Article Open

Purification of a factor that restores translation of vesicular stomatitis virus mRNA in extracts from poliovirus-infected HeLa cells

Abstract

It was previously shown that the poliovirus-induced inhibition of translation of capped mRNAs can be reversed by a protein found in preparations of the eukaryotic initiation factor eIF-4B [Rose, J. K., Trachsel, H., Leong, K. & Baltimore, D. (1978) Proc. Natl. Acad. Sci. USA 75, 2732--2736]. This "restoring factor" has now been purified from a high-salt wash of rabbit reticulocyte ribosomes by taking advantage of its tight association with factor eIF-3 at low salt concentrations. It did not copurify with the major M_r 80,000 polypeptide of eIF-4B preparations but did copurify with a M_r 24,000 polypeptide previously shown to bind to the cap structures of mRNAs [Sonenberg, N., Rupprecht, K. M., Hecht, S. M. & Shatkin, A. J. (1979) Proc. Natl. Acad. Sci. USA 76, 4345--4349]. Both the electrophoretic mobility and the tryptic peptide pattern of the restoring factor were indistinguishable from those of the cap-binding protein, and the restoring factor could be crosslinked to the 5'-terminal cap on mRNA. Thus, is appears that poliovirus inhibits cellular protein synthesis by inactivation of some crucial property of the cap-binding protein.

Additional Information

© 1980 National Academy of Sciences. Contributed by David Baltimore, October 22, 1979. The portion of this work that was done at Massachusetts Institute of Technology was supported by Grants AI-14566 (to J.K.R.) and Al-08388 (to D.B.) from the National Institute of Allergy and Infectious Diseases and Grant CA-14051 from the National Cancer Institute (core grant to S. E. Luria). D.B. is an American Cancer Society Professor of Microbiology. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.

Attached Files

Published - TRApnas80.pdf

Files

TRApnas80.pdf
Files (1.4 MB)
Name Size Download all
md5:fd8d23c6fe570e0296c5b0423e87c662
1.4 MB Preview Download

Additional details

Created:
August 22, 2023
Modified:
October 18, 2023