Published January 1982
| Published
Journal Article
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Amino-Terminal Sequence of the Tn3 Transposase Protein
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Abstract
The amino-terminal sequence of the Tn transposase protein was determined to be Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser.... This was determined both from an active transposase protein purified from a transposase overproducing mutant strain and from a hybrid transposase-β-galactosidase fusion protein. The amino acid sequence corresponded to the DNA sequence of the transposase gene beginning at an ATG initiation codon, as previously predicted from the analysis of transposase-β-galactosidase gene fusions.
Additional Information
© 1982 American Society for Microbiology. Received 20 July 1981; Accepted 16 September 1981. We thank Nicholas Cozzarelli for advice. This work was supported by Public Health Service grants GM 29067 and GM 06965 from the National Institute of General Medical Sciences to M.C. and L.H., respectively, and by National Science Foundation grant PCM 80-05599 to L.H. M.F. was supported by a fellowship from the Damon Runyon-Walter Winchell Cancer Fund (DRG-260-FT).Attached Files
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Additional details
- PMCID
- PMC216644
- Eprint ID
- 32484
- Resolver ID
- CaltechAUTHORS:20120716-143303838
- NIH
- GM 29067
- NIH
- GM 06965
- NSF
- PCM 80-05599
- Damon Runyon-Walter Winchell Cancer Fund
- DRG-260-FT
- Created
-
2012-07-16Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field