Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published March 1991 | Published
Journal Article Open

Silencing of the Expression of the Immunoglobulin Kappa Gene in Non-B Cells

Abstract

Although the activating factor NF-_K B can be present in the nucleus of many cell types, transcription and rearrangement of the immunoglobulin kappa chain gene is restricted to cells of the B lineage. Part of this specificity is determined by sequences within the major intron of the kappa gene that specifically silence gene expression in non-B cells (T cells and HeLa cells). These sequences are found in a 232-bp fragment located 5' of the NF-K B binding sequence of the enhancer. When this fragment is added back upstream of an active NF-_K B site, it specifically decreases the expression of a linked gene by more than 10-fold in activated T cells but it has no effect on expression in B cells. The kappa silencer region acts in an orientation- and distance-independent manner and appears to be composed of multiple negative elements. The kappa silencer may act to restrict transcription and rearrangement of the C_K locus to cells of the B lineage.

Additional Information

© 1991 American Society for Microbiology. Received 9 October 1990. Accepted 10 December 1990. We thank Astar Winoto, Mark S. Schlissel, and Ranjan Sen for helpful comments on the manuscript. This work was funded by a Special Fellowship of the Leukemia Society of America to J.W.P., Public Health Service grant GM-39458 to D.B., and Public Health Service grant GM-43874-01 from the National Institutes of Health to Ranjan Sen.

Attached Files

Published - PIEmcb91.pdf

Files

PIEmcb91.pdf
Files (1.3 MB)
Name Size Download all
md5:531579368d5472d014754f0d66c02eae
1.3 MB Preview Download

Additional details

Created:
August 19, 2023
Modified:
October 17, 2023