Electron tomography of cells
- Creators
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Gan, Lu
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Jensen, Grant J.
Abstract
The electron microscope has contributed deep insights into biological structure since its invention nearly 80 years ago. Advances in instrumentation and methodology in recent decades have now enabled electron tomography to become the highest resolution three-dimensional (3D) imaging technique available for unique objects such as cells. Cells can be imaged either plastic-embedded or frozen-hydrated. Then the series of projection images are aligned and back-projected to generate a 3D reconstruction or 'tomogram'. Here, we review how electron tomography has begun to reveal the molecular organization of cells and how the existing and upcoming technologies promise even greater insights into structural cell biology.
Additional Information
© 2011 Cambridge University Press. Published online: 15 November 2011. We thank Morgan Beeby, Ariane Briegel, Mark Ladinsky, Alasdair McDowall and Martin Pilhofer for comments and Karin Mallard for help with figures and editing. We apologize to all our colleagues whose work we could not highlight for space constraints. This work was supported in part by NIH grants GM066521 and GM094800B to G. J. J.Attached Files
Published - Gan2012p17514Q_Rev_Biophys.pdf
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Additional details
- Eprint ID
- 29858
- Resolver ID
- CaltechAUTHORS:20120327-091111234
- GM066521
- NIH
- GM094800B
- NIH
- Created
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2012-03-27Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field