Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly
- Creators
- Lu, Lei
- Ladinsky, Mark S.
- Kirchhausen, Tomas
Abstract
During mitosis, the nuclear envelope merges with the endoplasmic reticulum (ER), and nuclear pore complexes are disassembled. In a current model for reassembly after mitosis, the nuclear envelope forms by a reshaping of ER tubules. For the assembly of pores, two major models have been proposed. In the insertion model, nuclear pore complexes are embedded in the nuclear envelope after their formation. In the prepore model, nucleoporins assemble on the chromatin as an intermediate nuclear pore complex before nuclear envelope formation. Using live-cell imaging and electron microscope tomography, we find that the mitotic assembly of the nuclear envelope primarily originates from ER cisternae. Moreover, the nuclear pore complexes assemble only on the already formed nuclear envelope. Indeed, all the chromatin-associated Nup 107–160 complexes are in single units instead of assembled prepores. We therefore propose that the postmitotic nuclear envelope assembles directly from ER cisternae followed by membrane-dependent insertion of nuclear pore complexes.
Additional Information
© 2011 Lu et al. This article is distributed under the terms of an Attribution–Noncommercial– Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). Submitted: 10 December 2010. Accepted: 11 July 2011. Published August 8, 2011. We thank Eric Marino for maintaining the imaging resource used in this study, Brian Burke and Iain M. Cheeseman for generously providing reagents, Silvia Tacheva and Steeve Boulant for help setting up the experiments with Nup62, and members of our laboratory for helpful discussions. This work was supported by National Institutes of Health grants GM-075252 (to T. Kirchhausen), U54 AI057159 (New England Regional Center of Excellence in Biodefense and Emerging Infectious Disease, Core Imaging Facility), and SBS SUG M58080013 (to L. Lu).Attached Files
Published - Lu2011p15657J_Cell_Biol.pdf
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Additional details
- PMCID
- PMC3153650
- Eprint ID
- 25230
- Resolver ID
- CaltechAUTHORS:20110906-103158413
- GM-075252
- NIH
- U54 AI057159
- NIH
- SUG M58080013
- SBS
- Created
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2011-09-06Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field