Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published September 1, 2011 | Published + Supplemental Material
Journal Article Open

A Sox10 enhancer element common to the otic placode and neural crest is activated by tissue-specific paralogs

Abstract

The otic placode, a specialized region of ectoderm, gives rise to components of the inner ear and shares many characteristics with the neural crest, including expression of the key transcription factor Sox10. Here, we show that in avian embryos, a highly conserved cranial neural crest enhancer, Sox10E2, also controls the onset of Sox10 expression in the otic placode. Interestingly, we show that different combinations of paralogous transcription factors (Sox8, Pea3 and cMyb versus Sox9, Ets1 and cMyb) are required to mediate Sox10E2 activity in the ear and neural crest, respectively. Mutating their binding motifs within Sox10E2 greatly reduces enhancer activity in the ear. Moreover, simultaneous knockdown of Sox8, Pea3 and cMyb eliminates not only the enhancer-driven reporter expression, but also the onset of endogenous Sox10 expression in the ear. Rescue experiments confirm that the specific combination of Myb together with Sox8 and Pea3 is responsible for the onset of Sox10 expression in the otic placode, as opposed to Myb plus Sox9 and Ets1 for neural crest Sox10 expression. Whereas SUMOylation of Sox8 is not required for the initial onset of Sox10 expression, it is necessary for later otic vesicle formation. This new role of Sox8, Pea3 and cMyb in controlling Sox10 expression via a common otic/neural crest enhancer suggests an evolutionarily conserved function for the combination of paralogous transcription factors in these tissues of distinct embryological origin.

Additional Information

© 2011 The Company of Biologists Ltd. Accepted 20 June 2011. Published online before print July 20, 2011. We thank H. Kondoh for the pTK-EGFP reporter construct; Y. C. Cheng for full length Sox10; V. Lee for full length cSox8; C. LaBonne for XSox9K61R,365R; J.-P. Saint-Jeannet for full length XSox8; M. Dvorak for the cMyb antibody; M. Jones and J. Tan for technical help; M. Barembaum for full length cPea3; and C. Baker for helpful discussions. This project was supported by NIH grants HD037105 and DE16459. Deposited in PMC for release after 12 months.

Attached Files

Published - Betancur2011p15601Development.pdf

Supplemental Material - DEV057836FigS1.jpg

Supplemental Material - DEV057836FigS2.jpg

Files

DEV057836FigS1.jpg
Files (6.9 MB)
Name Size Download all
md5:423f4f7d736451c8cef17690b442b357
89.7 kB Preview Download
md5:42f7f5c56f5f7badad026732a5a30901
13.2 kB Preview Download
md5:4100a68e27634eed38e97cf492bbe38b
90.3 kB Preview Download
md5:73995f4f56991b3898f769f5a6a2560e
6.6 MB Preview Download
md5:0a6c43ce37ee3813aa3dbc737e81ea30
92.9 kB Preview Download

Additional details

Created:
August 22, 2023
Modified:
October 24, 2023