Published 2010
| public
Book Section - Chapter
Correlated Light and Electron Cryo-Microscopy
Abstract
Light and electron cryo-microscopy have each proven to be powerful tools to study biological structures in a near-native state. Light microscopy provides important localization information, while electron microscopy provides the resolution necessary to resolve fine structural details. Imaging the same sample by both light and electron cryo-microscopy is a powerful new approach that combines the strengths of both techniques to provide novel insights into cellular ultrastructure. In this chapter, the methods and instrumentation currently used to correlate light and electron cryo-microscopy are described in detail.
Additional Information
© 2010 Elsevier Inc. Available online 29 September 2010. We thank Dr. Alasdair McDowall for help with correlated FLM/ECM at Caltech. Work at Caltech was supported by the HHMI, NIH grants R01 AI067548 and P01 GM066521 to GJJ, and gifts from the Gordon and Betty Moore Foundation. Work at Boulder was supported by NIH Research Resource Grant #RR00592 to A. Hoenger. Work at the Max-Planck-Institute of Biochemistry in Martinsried was supported by the European Commission's 7th Framework Programme (grant agreement HEALTH-F4-2008-201648/ PROSPECTS).Additional details
- Eprint ID
- 22547
- DOI
- 10.1016/S0076-6879(10)81013-4
- Resolver ID
- CaltechAUTHORS:20110228-111002641
- Howard Hughes Medical Institute (HHMI)
- R01 AI067548
- NIH
- P01 GM066521
- NIH
- Gordon and Betty Moore Foundation
- RR00592
- NIH
- HEALTH-F4-2008-201648/PROSPECTS
- European Commission
- Created
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2011-03-03Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field
- Series Name
- Methods in Enzymology
- Series Volume or Issue Number
- 481