Wide field-of-view microscope based on holographic focus grid illumination
Abstract
We have developed a new microscopy design that can achieve wide field-of-view (FOV) imaging and yet possesses resolution that is comparable to a conventional microscope. In our design, the sample is illuminated by a holographically projected light-spot grid. We acquire images by translating the sample across the grid and detecting the transmissions. We have built a prototype system with an FOV of 6mm×5mm and acquisition time of 2.5s. The resolution is fundamentally limited by the spot size—our demonstrated average FWHM spot diameter was 0.74μm. We demonstrate the prototype by imaging a U.S. Air Force target and a lily anther. This technology is scalable and represents a cost-effective way to implement wide FOV microscopy systems
Additional Information
© 2010 Optical Society of America. Received March 22, 2010; revised May 19, 2010; accepted May 29, 2010; posted June 7, 2010 (Doc. ID 125762); published June 23, 2010. This work is supported by Department of Defense grant W81XWH-09-1-0051. The authors acknowledge Richard Cote and Ram Datar from the University of Miami for helpful discussions.Attached Files
Published - Wu2010p10606Opt_Lett.pdf
Files
| Name | Size | Download all |
|---|---|---|
|
md5:6966dd94ee14940d76a3dda5234f8368
|
348.9 kB | Preview Download |
Additional details
- Eprint ID
- 19003
- Resolver ID
- CaltechAUTHORS:20100712-153457723
- W81XWH-09-1-0051
- Department of Defense
- Created
-
2010-08-04Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field