A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators

Creators: Liu, Zhenfeng; Walton, Troy A.; Rees, Douglas C.

Abstract

Several archaeal mechanosensitive (MS) channels have been reported, including one from Thermoplasma volcanium designated MscTV. Here, we report the crystal structure of MscTV at 1.6-Å resolution. Unexpectedly, MscTV was found to be a water-soluble protein exhibiting a winged helix-turn-helix (wHTH) motif, which is the signature of the MarR (multiple antibiotic resistance regulator) family of transcriptional regulators. A cell-based osmotic downshock functional assay demonstrated that MscTV was unable to protect a knockout strain of Escherichia coli from hypoosmotic shock, further indicating that it does not function as a MS channel. We propose this protein be renamed MLPTv for MarR-like protein from T. volcanium.

Additional Information

© 2010 The Protein Society. Received: 27 December 2009; Revised: 23 January 2010; Accepted: 25 January 2010. The authors thank A. Shih for cloning work, Y. Poon and J. Lai for protocols of osmotic downshock assay and lysogenizing MJF465 cells with kDE3, Y. Liu and M. Shahgholi for the mass spectrometry analysis, B. Martinac, H. Pinkett, and C. Gandhi for discussions, P. Blount for the MJF465 cells, and the staff at SSRL for technical support during data collection. The authors acknowledge the Gordon and Betty Moore Foundation for support of the Molecular Observatory at Caltech. Operations at SSRL are supported by the U.S. Department of Energy and NIH. D. C. R. is an Investigator in the Howard Hughes Medical Institute.

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A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators

Abstract

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