Design, modeling and synthesis of an in vitro transcription rate regulatory circuit
Abstract
This paper describes the design, modeling and realization of a synthetic in vitro circuit that aims at regulating the rate of mRNA transcription. Two DNA templates are designed to interact through their transcripts, creating negative feedback loops that will equate their transcription rates at steady state. A mathematical model is developed for this circuit, consisting of a set of ODEs derived from the mass action laws and Michaelis-Menten kinetics involving all the present chemical species. The DNA strands were accordingly designed, following thermodynamics principles and minimizing unwanted interactions. Preliminary experimental results show that the circuit is performing the expected task, by matching at steady state the transcription rates of the two DNA templates.
Additional Information
© 2008 IEEE. Issue Date: 11-13 June 2008; Date of Current Version: 05 August 2008. Research supported in part by the Institute for Collaborative Biotechnologies through grant DAAD19-03-D-0004 from the U.S. Army Research Office. All the experiments presented in this paper were done in Erik Winfree's laboratory. The authors would like to thank Erik Winfree, Jongmin Kim and all the members of the DNA group at Caltech for their helpful advise during the development of this project.Attached Files
Published - Franco2008p84452009_American_Control_Conference_Vols_1-9.pdf
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Additional details
- Eprint ID
- 18251
- Resolver ID
- CaltechAUTHORS:20100512-105503658
- DAAD19-03-D-0004
- Army Research Office (ARO)
- Created
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2010-06-24Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field