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Published January 19, 2010 | Supplemental Material + Published
Journal Article Open

Mechanism of active targeting in solid tumors with transferrin-containing gold nanoparticles

Abstract

PEGylated gold nanoparticles are decorated with various amounts of human transferrin (Tf) to give a series of Tf-targeted particles with near-constant size and electrokinetic potential. The effects of Tf content on nanoparticle tumor targeting were investigated in mice bearing s.c. Neuro2A tumors. Quantitative biodistributions of the nanoparticles 24 h after i.v. tail-vein injections show that the nanoparticle accumulations in the tumors and other organs are independent of Tf. However, the nanoparticle localizations within a particular organ are influenced by the Tf content. In tumor tissue, the content of targeting ligands significantly influences the number of nanoparticles localized within the cancer cells. In liver tissue, high Tf content leads to small amounts of the nanoparticles residing in hepatocytes, whereas most nanoparticles remain in nonparenchymal cells. These results suggest that targeted nanoparticles can provide greater intracellular delivery of therapeutic agents to the cancer cells within solid tumors than their nontargeted analogs.

Additional Information

© 2010 by the National Academy of Sciences. Freely available online through the PNAS open access option. Contributed by Mark E. Davis, December 8, 2009 (sent for review November 24, 2009). Published online before print December 29, 2009. We thank Debbie Guerrero, Siva Wu, Jennifer Bower (House Ear Institute), Carol Garland, and Nathan Dalleska (California Institute of Technology) for advice on histology, transmission electron microscopy, and ICP-MS. This work was supported by National Cancer Institute Grant CA 119347 and National Institutes of Health Grant R01 EB004657. Author contributions: C.H.J.C. and M.E.D. designed research; C.H.J.C. and C.A.A. performed research; C.A.A., P.W., and M.E.D. analyzed data; and C.H.J.C. and M.E.D. wrote the paper.

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Published - Choi2010p7056P_Natl_Acad_Sci_Usa.pdf

Supplemental Material - pnas.200914140SI.pdf

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