Published November 2, 2009 | Accepted Version + Supplemental Material
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Generation of Surface-Bound Multicomponent Protein Gradients

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Abstract

Spatial control of bioactive ligands is achieved by integrating microfluidics and protein engineering. The proteins of interest are mixed in a gradient generator and immobilized on artificial polypeptide scaffolds through the strong association of heterodimeric ZE/ZR leucine zipper pairs. Protein densities and gradient shapes are easily controlled and varied in this method.

Additional Information

© 2009 WILEY. Received: 28 August 2009; published Online: 24 Sep 2009. This work was supported by the NSF-sponsored Center for the Science and Engineering of Materials at the California Institute of Technology, and by NIH grant GM62523.

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Supplemental Material - sm001.pdf

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