Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published January 5, 2009 | Accepted Version + Supplemental Material
Journal Article Open

Biosynthesis and Stability of Coiled-Coil Peptides Containing (2S,4R)-5,5,5-Trifluoroleucine and (2S,4S)-5,5,5-Trifluoroleucine

Abstract

We report the effects of 5,5,5-trifluoroleucine (TFL) stereochemistry on coiled-coil peptide biosynthesis and stability. We demonstrate that two diastereoisomers of TFL are activated and incorporated into peptides expressed in E. coli. Coiled-coil homodimers of these peptides exhibited increased stability. An equimolar mixture of the two fluorinated peptides formed a heterodimer of modestly enhanced thermal stability relative to the homodimers.

Additional Information

© 2009 Wiley. This work was supported by the NIH grants GM62523 and 5FM GM67375-2 (D.A.T. and J.K.M.), and GM65500 (K.K.), NSF graduate fellowship (S.S.), GAANN fellowship (G.A.C.), and a NSF CAREER award (K.K.).

Attached Files

Accepted Version - nihms327142.pdf

Supplemental Material - sm001.pdf

Files

nihms327142.pdf
Files (667.3 kB)
Name Size Download all
md5:aa3478a8ddcdc1ccc45639f4f840a93b
614.4 kB Preview Download
md5:ef8432817af792c6e9c472ff4bf1dcc3
52.9 kB Preview Download

Additional details

Created:
August 22, 2023
Modified:
October 19, 2023