Published February 16, 2009
| Accepted Version + Supplemental Material
Journal Article
Open
High-Potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa Azurins
Chicago
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Abstract
Site-directed mutagenesis of Pseudomonas aeruginosa azurin C112D at the M121 position has afforded a series of proteins with elevated Cu^(II/I) reduction potentials relative to the CuII aquo ion. The high potential and low axial hyperfine splitting (Cu^(II) electron paramagnetic resonance A|) of the C112D/M121L protein are remarkably similar to features normally associated with type 1 copper centers.
Additional Information
© 2009 American Chemical Society. Received December 3, 2008. We thank Yuling Sheng and Matthew R. Hartings for assistance and Israel Pecht for useful discussions. This work was supported by the NSF (Grant CHE-0553150 to H.B.G. and J.R.W.; graduate fellowship to K.M.L.), GCEP (Stanford), and NIH (Grant DK019038 to H.B.G.)Attached Files
Accepted Version - nihms86677.pdf
Supplemental Material - ic802322e_si_001.pdf
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Additional details
- PMCID
- PMC2765487
- Eprint ID
- 15723
- Resolver ID
- CaltechAUTHORS:20090910-101427356
- NSF
- CHE-0553150
- NIH
- DK019038
- Created
-
2009-09-10Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field