Published January 14, 2009
| Supplemental Material + Published
Journal Article
Open
^1H{^(19)F} NOE NMR Structural Signatures of the Insulin R_6 Hexamer: Evidence of a Capped HisB10 Site in Aryl- and Arylacryloyl-carboxylate Complexes
Abstract
New and improved insulin: ^1H{^(19)F} NOE NMR difference spectra for CF_3-substituted aromatic carboxylates bound at the HisB10 sites of the R_6 human insulin (HI) hexamer show strong NOEs between the CF_3 groups and the LeuB6, AsnB3, and PheB1 sidechains. The NOEs and structural modeling establish that these carboxylates form closed complexes with the HisB10 site capped by the PheB1 rings.
Additional Information
© 2009 WILEY-VCH Verlag GmbH & Co. Received: November 12, 2008 Published online on January 14, 2009. We thank Novo Nordisk for the wild-type human and variant insulin, and for a gift to M.F.D. in support of this work.Attached Files
Published - Keidel2009p64910.1002cbic.200800746.pdf
Supplemental Material - sm001.pdf
Files
Keidel2009p64910.1002cbic.200800746.pdf
Files
(881.0 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:a5b14775eebe9841ae0b4b5a79b93161
|
503.1 kB | Preview Download |
|
md5:8265bdb5df1ec24932e7fccc1e5204e3
|
377.9 kB | Preview Download |
Additional details
- Eprint ID
- 15713
- Resolver ID
- CaltechAUTHORS:20090910-084752456
- Novo Nordisk
- Created
-
2009-09-10Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field