Fluorescein Redirects a Ruthenium−Octaarginine Conjugate to the Nucleus
- Creators
- Puckett, Cindy A.
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Barton, Jacqueline K.
Abstract
The cellular uptake and localization of a Ru−octaarginine conjugate with and without an appended fluorescein are compared. The inherent luminescence of the Ru(II) dipyridophenazine complex allows observation of its uptake without the addition of a fluorophore. Ru−octaarginine−fluorescein stains the cytosol, nuclei, and nucleoli of HeLa cells under conditions where the Ru−octaarginine conjugate without fluorescein shows only punctate cytoplasmic labeling. At higher concentrations, however, Ru−octaarginine without the fluorescein tag does exhibit cytoplasmic, nuclear, and nucleolar staining. Attaching fluorescein to Ru−octaarginine lowers the threshold concentration required for diffuse cytoplasmic labeling and nuclear entry. Hence, the localization of the fluorophore-bound peptide cannot serve as a proxy for that of the free peptide.
Additional Information
Copyright © 2009 American Chemical Society. Received March 30, 2009; Publication Date (Web): June 8, 2009. We are grateful to the NIH (GM33309) and to the Caltech Biological Imaging Center.Attached Files
Accepted Version - nihms123170.pdf
Supplemental Material - Puckettja9025165_si_001.pdf
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Additional details
- PMCID
- PMC2747598
- Eprint ID
- 15527
- DOI
- 10.1021/ja9025165
- Resolver ID
- CaltechAUTHORS:20090901-133357644
- NIH
- GM33309
- Created
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2009-09-09Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field