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Published August 1, 2009 | Supplemental Material + Published
Journal Article Open

Cisternal Organization of the Endoplasmic Reticulum during Mitosis

Abstract

The endoplasmic reticulum (ER) of animal cells is a single, dynamic, and continuous membrane network of interconnected cisternae and tubules spread out throughout the cytosol in direct contact with the nuclear envelope. During mitosis, the nuclear envelope undergoes a major rearrangement, as it rapidly partitions its membrane-bound contents into the ER. It is therefore of great interest to determine whether any major transformation in the architecture of the ER also occurs during cell division. We present structural evidence, from rapid, live-cell, three-dimensional imaging with confirmation from high-resolution electron microscopy tomography of samples preserved by high-pressure freezing and freeze substitution, unambiguously showing that from prometaphase to telophase of mammalian cells, most of the ER is organized as extended cisternae, with a very small fraction remaining organized as tubules. In contrast, during interphase, the ER displays the familiar reticular network of convolved cisternae linked to tubules.

Additional Information

Copyright © 2009 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,. the appearance of) the edited manuscript in an online issue of MBoC, the nonexclusive right to copy, distribute, or display the manuscript subject to the terms of the Creative Commons–Noncommercial–Share Alike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0). Submitted April 24, 2009; Revised May 21, 2009; Accepted May 26, 2009. This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E09-04-0327) on June 3, 2009. This work is funded by National Institutes of Health grant R01 GM-075252 (to T .K.). We thank Eric Marino for assistance in maintaining the microscope facility in the Kirchhausen laboratory and Tom Rapoport, Randall King, Jennifer Lippincott-Schwartz, and Howard Worman for generously providing reagents.

Attached Files

Published - Lu2009p5595Mol_Biol_Cell.pdf

Published - Smov03.mov

Supplemental Material - 1-1.pdf

Supplemental Material - Smov01.mov

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Created:
August 20, 2023
Modified:
October 18, 2023