Floral stem cell termination involves the direct regulation of AGAMOUS by PERIANTHIA
Abstract
In Arabidopsis, the population of stem cells present in young flower buds is lost after the production of a fixed number of floral organs. The precisely timed repression of the stem cell identity gene WUSCHEL (WUS) by the floral homeotic protein AGAMOUS (AG) is a key part of this process. In this study, we report on the identification of a novel input into the process of floral stem cell regulation. We use genetics and chromatin immunoprecipitation assays to demonstrate that the bZIP transcription factor PERIANTHIA (PAN) plays a role in regulating stem cell fate by directly controlling AG expression and suggest that this activity is spatially restricted to the centermost region of the AG expression domain. These results suggest that the termination of floral stem cell fate is a multiply redundant process involving loci with unrelated floral patterning functions.
Additional Information
© 2009 The Company of Biologists Ltd. Accepted 16 March 2009. We thank J. Lohmann for sharing unpublished results; Arnavaz Garda, Alexis Lacroix, Claudia Bardoux and Hervé Leyral for technical assistance; Ioan Negrutiu, Christophe Trehin, Nathanaël Prunet and Olivier Hamant for useful discussions and comments on the manuscript; Ioan Negrutiu for sharing unpublished results and seeds; and John Bowman and Marty Yanofsky for communicating unpublished results. This work was supported by a Caltech Division of Biology postdoctoral fellowship (to P.D.), a Marie Curie Incoming International Fellowship IIF-022002 (to P.D.), the EU Marie Curie SY-STEM Network (to J.T.) and a NSF Grant IOS-0544915 (to E.M.M.).Attached Files
Published - Das2009p2415Development.pdf
Supplemental Material - Das2009p2415Development_supp1.pdf
Supplemental Material - Das2009p2415Development_supp2.pdf
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Supplemental Material - Das2009p2415Development_supp7.pdf
Supplemental Material - Das2009p2415Development_supp8.pdf
Supplemental Material - Das2009p2415Development_supp9.pdf
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Additional details
- Eprint ID
- 14377
- Resolver ID
- CaltechAUTHORS:20090609-104852613
- Caltech Division of Biology
- IIF-022002
- Marie Curie Fellowship
- IOS-0544915
- NSF
- Created
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2009-07-14Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field