Immunoglobulin heavy chain gene rearrangement and transcription in murine T cell hybrids and T lymphomas

Abstract

We have examined the arrangement of immunoglobulin heavy chain constant (CH) and joining (JH) region genes in murine T cell hybrid lines and in T lymphomas. CH genes derived from both parental cell types were present in all hybrids for which polymorphism in sequences flanking CH genes permitted us to distinguish parental CH genes. All T lymphomas and T cell hybrids retained the Cα gene in germ-line configuration and all but one cell line had germ-line Cµ genes. Novel DNA fragments reactive with JH probes were observed in six of nine T cell hybrids, as well as in two T lymphomas, WEH17.1 and YAC-1, but not in the fusion parent, BW5147. No RNA homologous to Cγ2b, Cα, or λ genes was detected in any of the T cell lines. T cell lines contained poly(A)+ RNA homologous to a Cµ cDNA probe. More importantly, in several cell lines the Cµ RNAs were associated with membrane-bound polyribosomes. These results suggest that both JH rearrangements and Cµ RNA production occur in at least some mature, antigen-specific T cells. They may therefore reflect events in normal T cell development and function related to those involved in the generation of the T receptor for antigen.

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