Published July 1972
| Published
Journal Article
Open
Deletion mutants of bacteriophage phiX174
Abstract
Mutants of bacteriophage phiX174 have been isolated that are less dense than wild-type phiX particles in CsCl. When mutant viral (+) strand DNA and wild-type complementary (-) strand DNA are hybridized, the resulting duplex molecules have single-stranded loops characteristic of wild-type-deletion heteroduplexes. The mutant bacteriophages fail to complement phiX amber mutants in cistron E. We conclude that the mutant viruses have deleted approximately 7% of the phiX genome in the region of cistron E.
Additional Information
© 1972 by the National Academy of Sciences. Contributed by Robert L. Sinsheimer, April 24, 1972. We are deeply indebted to J.S. Kim, P.A. Sharp, and N. Davidson for their patient instruction in the preparation of samples for electron microscopy and for their suggestions in the writing of this manuscript. This research was supported by USPHS Grant GM-13554, USPHS Training Grant GM-00086, and by a National Science Foundation Graduate Fellowship.Attached Files
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Additional details
- PMCID
- PMC426829
- Eprint ID
- 9479
- Resolver ID
- CaltechAUTHORS:ZUCpnas72
- GM 13554
- U.S. Public Health Service (USPHS)
- GM-00086
- U.S. Public Health Service (USPHS)
- NSF Graduate Research Fellowship
- Created
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2008-01-23Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field