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Published January 17, 2000 | public
Journal Article Open

An apertureless near-field microscope for fluorescence imaging

Abstract

We describe an apertureless near field microscope for imaging fluorescent samples. Optical contrast is generated by exploiting fluorescent quenching near a metallized atomic force microscope tip. This microscope has been used to image fluorescent latex beads with subdiffraction limit resolution. The use of fluorescence allows us to prove that the contrast mechanism is indeed spectroscopic in origin.

Additional Information

©2000 American Institute of Physics. (Received 18 August 1999; accepted 16 November 1999) The authors thank Pierre Barritault and Francisco Guzman for contributions in the early stages of this work and Jeff Kimble for the generous loan of equipment. This work was supported in part by the Keck Foundation. G.L. had partial financial support from the Fonds FCAR.

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