Methidiumpropyl-EDTA•Fe(II) and DNase I footprinting report different small molecule binding site sizes on DNA
- Creators
- Van Dyke, Michael W.
-
Dervan, Peter B.
Abstract
DNase I and MPE.Fe (II) footprinting both employ partial cleavage of ligand-protected DNA restriction fragments and Maxam-Gilbert sequencing gel methods of analysis. One method utilizes the enzyme, DNase I, as the DNA cleaving agent while the other employs the synthetic molecule, methidium-propyl-EDTA (MPE). For actinomycin D, chromomycin A3 and distamycin A, DNase I footprinting reports larger binding site sizes than MPE.Fe (II). DNase I footprinting appears more sensitive for weakly bound sites. MPE.Fe (II) footprinting appears more accurate in determining the actual size and location of the binding sites for small molecules on DNA, especially in cases where several small molecules are closely spaced on the DNA. MPE.Fe (II) and DNase I report the same sequence and binding site size for lac repressor protein on operator DNA.
Additional Information
© 1983 IRL Press Limited, Oxford, England. Received 4 April 1983; Revised 5 July 1983; Accepted 21 July 1983. We are grateful to the National Institutes of Health for grant support (GM-27681) and a National Research Service Award (GM-07616) to MWV. Division of Chemistry and Chemical Engineering, Contribution Number 6797, California Institute of Technology, Pasadena, CA, 91125, USAAttached Files
Published - VANnar83.pdf
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Additional details
- PMCID
- PMC326297
- Eprint ID
- 4142
- Resolver ID
- CaltechAUTHORS:VANnar83
- GM-27681
- NIH
- GM-07616
- NIH Predoctoral Fellowship
- Created
-
2006-08-07Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field
- Other Numbering System Name
- Caltech Division of Chemistry and Chemical Engineering
- Other Numbering System Identifier
- 6797