Amphoteric behavior of complex systems. II. Titration of sulfanilic acid-glycine mixtures

Abstract

It is the purpose of this and the following paper to test portions of the theory in a more quantitative manner, and in this paper the mutual action of two ampholytes, glycine and sulfanilic acid, is studied by means of titration over a wide pH range. The latter substance was chosen to correspond to the strongly acid nucleic acid in living matter. That it is amphoteric, with a measurable basic ionization constant, is shown in the fourth paper of the series. In fact it has an isoelectric point at a distinctly higher pH, 1.25, than that (0.7) of nucleic acid (2). The glycine used was a preparation from the Eastman Kodak Company; and the sulfanilic acid was a Merck C.P. grade, which was reprecipitated from alkaline solution by hydrochloric acid, washed, and dried.

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