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Published March 11, 1982 | public
Journal Article Open

Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids

Seed, Brian

Abstract

We describe the synthesis of a family of arylamine-substituted papers which can be diazotized and coupled with nucleic acids. The synthesis is simple and uses readily obtainable starting materials. A partial characterization of the nucleic acid binding activity is reported, as well as a demonstration of the utility of the activated paper for the detection of electrophoretically separated RNA by blot transfer and hybridization.

Additional Information

Copyright © 1982 Oxford University Press. Received November 10, 1981. Revised February 5, 1982. Accepted February 5, 1982. I would like to thank the many people who shared their experiences with me, traumatic and otherwise; and among them particularly Jim Casey, Chien Yueh-Hsieh, David Goldberg, Ross Hardison, and Liz Lacy. I would also like to thank Tom Maniatis for laboratory space, and my parents for support.

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