The synthesis of hemoglobin in a cell-free system
Abstract
The microsomal particles (1) have been implicated as the major sites of protein synthesis within the cells of a variety of tissues. The studies of Borsook, Zamecnik, Hultin, and others, with intact animals and various types of whole cell systems (see review by Askonas et al. (2)), as well as electron-microscope studies (3), provided the original evidence for this conclusion. Zamecnik and Keller (4) have, in addition, developed cell-free systems which incorporate C14-labeled amino acids into protein. These include microsomes as well as various soluble enzymes (5). Further studies have partially defined the intermediate stages involved (6). However, it has not yet been possible to equate incorporation of labeled amino acid into protein with actual protein synthesis (see review by Campbell (7)).
Additional Information
© 1958 by the National Academy of Sciences. Communicated by James Bonner, August 22, 1958. We wish to express our appreciation to Dr. Henry Borsook, whose encouragement and numerous contributions in this field have greatly aided these studies. It is a pleasure to thank Dr. James Bonner and Dr. Howard Dintzis for many stimulating discussions. This work was done during the tenure of an Established Investigatorship of the American Heart Association [R.S.]. These studies were supported by grants from the National Science Foundation and the American Heart Association. [H.L. was] [s]upported by grant No. C-1624 of the United States Public Health Service.Files
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Additional details
- Eprint ID
- 10942
- Resolver ID
- CaltechAUTHORS:SCHWpnas58b
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2008-06-20Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field